cd66 fitc  (Miltenyi Biotec)

Journal: Frontiers in Immunology

Article Title: Circulating mucosal-like IgA responses increase with severity of Puumala orthohantavirus-caused hemorrhagic fever with renal syndrome

doi: 10.3389/fimmu.2024.1480041

Figure Lengend Snippet: PBMC flow cytometry antibody panel.

Article Snippet: CD66 , FITC , 6g5j , Immunotools.

Techniques: Flow Cytometry

Journal: PLoS ONE

Article Title: Shiga Toxin and Lipopolysaccharide Induce Platelet-Leukocyte Aggregates and Tissue Factor Release, a Thrombotic Mechanism in Hemolytic Uremic Syndrome

doi: 10.1371/journal.pone.0006990

Figure Lengend Snippet: (A) The neutrophil and monocyte population were identified in whole blood by their characteristic size and granularity. In the monocyte gate 98% of the cells were positive for the monocyte marker CD38:FITC and 99% of the cells in the neutrophil gate were CD66:FITC positive showing the accuracy of the identification of cells by forward and side scatter. (B) Monocytes or neutrophils in complex with platelets (gate 2), were identified by binding of CD38:FITC or CD66:FITC (FL1) and the platelet specific antibody CD42b:RPE-Cy5 (FL3). Cells in gate 3 represent platelet-free monocytes or neutrophils. (C) Surface bound tissue factor was identified by binding of CD142:RPE and CD38:FITC or CD66:FITC (FL2 vs. FL1). (D) Percentage of positive cells was calculated by subtraction of the negative control antibody.

Article Snippet: Microparticles were isolated as previously described and incubated with Annexin V-Cy5 (1∶100, BD Biosciences) , mouse anti-human tissue factor CD142:RPE and mouse anti-human CD42b:FITC or mouse anti-human CD38:FITC, alternatively mouse anti-human CD66:FITC, simultaneously or isotype controls IgG 1 :FITC and IgG 1 :PE (all from BD Biosciences).

Techniques: Marker, Binding Assay, Negative Control

Journal: PLoS ONE

Article Title: Shiga Toxin and Lipopolysaccharide Induce Platelet-Leukocyte Aggregates and Tissue Factor Release, a Thrombotic Mechanism in Hemolytic Uremic Syndrome

doi: 10.1371/journal.pone.0006990

Figure Lengend Snippet: (A) Incubation of PBS-treated whole blood at 37°C for 4 h induced a slight increase in platelet-monocyte (A) and platelet-neutrophil (B) aggregate formation compared to baseline levels. Baseline levels of platelet-monocyte aggregates were 10% (range 5–17%, 7 experiments) and platelet-neutrophil aggregates 6% (range 2–13%, 7 experiments). Incubation of whole blood with Stx2 and/or O157LPS (0.5 µg/mL) induced predominantly the formation of platelet-monocyte aggregates. (C) Incubation of whole blood with O157LPS (1 µg/mL) at 37°C for 4 h induced significantly more aggregate formation between platelets and monocytes in comparison to the other LPS serotypes tested while no significant differences, between LPS serotypes, were observed in platelet-neutrophil (D) aggregate formation. Results are expressed as the percentage of the monocyte or neutrophil population that was positive for CD38:FITC or CD66:FITC as well as the platelet specific marker CD42b:RPE-Cy5. Data are expressed as mean±standard deviation (n = 10 experiments), ** denotes P <0.01 when comparing aggregate formation in whole blood incubated with a stimulant and PBS-treated whole blood and * denotes P <0.05, comparing aggregate formation in whole blood incubated with O157LPS with those incubated with O103, O111, O121, or O111:B4LPS. NS; indicates not significant.

Article Snippet: Microparticles were isolated as previously described and incubated with Annexin V-Cy5 (1∶100, BD Biosciences) , mouse anti-human tissue factor CD142:RPE and mouse anti-human CD42b:FITC or mouse anti-human CD38:FITC, alternatively mouse anti-human CD66:FITC, simultaneously or isotype controls IgG 1 :FITC and IgG 1 :PE (all from BD Biosciences).

Techniques: Incubation, Marker, Standard Deviation